417 Evaluation of a novel skin emollient on skin lipidome and lipids organization

The stratum corneum (SC) matrix is composed of free fatty acids, cholesterol and ceramides (CERs), which play a key role in the skin barrier function. effect glycerol/petrolatum-based emollient (G/P-emollient) cream on lipid profile SC reconstructed human epithelial (RHE) model was measured. spatial organization intercellular studied using X-ray diffraction. inter-bilayer distance multi-lamellar structures lattice type were analyzed small-angle scattering (SAXS) wide-angle (WAXS), respectively. crystallized components G/P-emollient are lipids, mainly packed hexagonal orthorhombic lattices, similar to structure. penetrated but did not alter WAXS profile. It increased long chain length CERs, all acyl-CERs, acyl-CER classes (CER EOS, EOH, EOP, EOdS) total CER classes, decreased short C34 for hydroxylated non-hydroxylated CERs. had positive impact [S] [P] forms (increased [NP]/[NS] [AP]/[AS] ratios), indicating it could help reduce relative feedback mechanism observed inflammatory pathologies e.g., atopic dermatitis. CER[NP], dry skin. In conclusion, can balance negative by modifying balancing levels ratios, improving Importantly, structure mimics that lower layers without compromising its lamellar